CapNMR Data on 10% 2-ethyl-1-indanone: 1D & 2D NMR
Important Note: These are examples from a single working system; pulse width, power level, and other parameters
Analyte: 2-ethyl-1-indanone
FW: 160.21 g/mol
Density: 1.038 g/mL
Sample: 10% 2-ethyl-1-indanone in deuterated chloroform
Total Sample Volume: 5 µL
Total Analyte Mass: 519 µg (3.2 µmol)
Sample Entry: Direct manual injection
Note: This high-mass analysis is an excessive amount of sample for the CapNMR probe.
For a more appropriate low-mass analysis, please see our 50 µg (1%) 2-ethyl-1-indanone dataset.
Injection Method
A Hamilton Gastight 25-µL syringe was filled with clean CDCl3 solvent and used to rinse the probe. Then, 5-µL of 10% 2-ethyl-1-indanone (519 µg; 3.2 µmol; v/v in CDCl3) was drawn into the syringe and injected into the probe. Using the same syringe, 12 µL of clean CDCl3 was picked up and injected into the probe to push the 5-µL sample into the NMR flow cell of the CapNMR probe. The 12-µL Push Volume was calibrated in advance of sample injection.
Screenshots
All of the screenshots shown below on this page are from a Varian Inova console running Varian VnmrJ 2.1B software on a Unix workstation. A 600 MHz Oxford unshielded magnet was employed. (For more information on the Varian VnmrJ software, see http://www.varianinc.com/
Here is a list of Technical Bulletins that describe the setup and specific parameters for a common suite of experiments on the 5-µL of 10% 2-ethyl-1-indanone.
Tech Bulletin C0010 - Proton for CapNMR
Tech Bulletin C0020 - Direct Carbon Detection for CapNMR
Tech Bulletin C0030 - gCOSY for CapNMR
Tech Bulletin C0040 - TOCSY for CapNMR
Tech Bulletin C0050 - NOESY for CapNMR
Tech Bulletin C0060 - gHSQC for CapNMR
Tech Bulletin C0070 - gHMBC for CapNMR
[Click on the parameter set for a larger higher resolution image]
Proton (1H) (55 seconds)
Direct Carbon (13C) (2 hours)
Full Data Set (Background Subtracted)
Background Peaks Region after Subtraction
gCOSY (Homonuclear 1H-1H) (10 minutes 37 seconds)
Tocsy (Through Multiple-Bond 1H-1H) (1 hour 48 minutes)
Noesy (Through Space 1H-1H) (2 hours 32 minutes)
gHSQC (Single Bond 1H-13C) (3 hours 56 minutes)
Ghmbc (Multiple Bond 1H-13C) (5 hours 56 minutes)
